大鼠内皮素1(ET-1)酶联免疫吸附检测试剂盒

英文名称	Rat ET-1(Endothelin1) ELISA Kit
别名	ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1

种属	Rat
检测类型	Sandwich
灵敏度	2.63 pg/mL
检测范围	7.82-500 pg/mL
样本类型	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Endocrinology;Cardiovascular biology;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P22387

标准曲线图

【仅供科学研究使用】

相关产品:

产品名称	点击数
鸡补体成分3(C3)酶联免疫吸附检测试剂盒	1160
犬肿瘤坏死因子α(TNFa)酶联免疫吸附检测试剂盒	1384
小鼠玻连蛋白(VTN)酶联免疫吸附检测试剂盒	1105
小鼠玻连蛋白(VTN)微量上样酶联免疫吸附检测试剂盒	911
小鼠载脂蛋白H(APOH)酶联免疫吸附检测试剂盒	1076
小鼠载脂蛋白H(APOH)微量上样酶联免疫吸附检测试剂盒	1047
大鼠载脂蛋白H(APOH)酶联免疫吸附检测试剂盒	1571
大鼠载脂蛋白H(APOH)微量上样酶联免疫吸附检测试剂盒	1378
大鼠补体成分2(C2)酶联免疫吸附检测试剂盒	1059
大鼠补体成分2(C2)微量上样酶联免疫吸附检测试剂盒	1304
小鼠补体1q(C1q)酶联免疫吸附检测试剂盒	1225
小鼠补体1q(C1q)微量上样酶联免疫吸附检测试剂盒	1740
小鼠α1-微球蛋白(a1M)酶联免疫吸附检测试剂盒	1166
大鼠α1-酸性糖蛋白(a1AGP)酶联免疫吸附检测试剂盒	1115
大鼠补体成分3(C3)酶联免疫吸附检测试剂盒	1167
大鼠补体1q(C1q)酶联免疫吸附检测试剂盒	1465
大鼠补体1q(C1q)微量上样酶联免疫吸附检测试剂盒	1600
大鼠α1-微球蛋白(a1M)酶联免疫吸附检测试剂盒	1752
小鼠巨噬细胞来源趋化因子(MDC)酶联免疫吸附检测试剂盒	762
兔结合珠蛋白(HP)酶联免疫吸附检测试剂盒	1450