人内皮素1(ET-1)酶联免疫吸附检测试剂盒

英文名称	Human ET-1(Endothelin1) ELISA Kit
别名	ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1

种属	Human
检测类型	Sandwich
灵敏度	2.73 pg/mL
检测范围	7.82-500 pg/mL
样本类型	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长	3.5h

研究领域	Endocrinology;Cardiovascular biology;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ET-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ET-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ET-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P05305

标准曲线图

【仅供科学研究使用】

相关产品:

产品名称	点击数
猴白介素10(IL10)酶联免疫吸附检测试剂盒	1713
猴白介素1β(IL1b)酶联免疫吸附检测试剂盒	2046
小鼠Adropin蛋白(AD)酶联免疫吸附检测试剂盒	1430
绵羊白介素1α(IL1a)酶联免疫吸附检测试剂盒	1468
绵羊白介素8(IL8)酶联免疫吸附检测试剂盒	1997
绵羊白介素17(IL17)酶联免疫吸附检测试剂盒	2297
绵羊白介素1β(IL1b)酶联免疫吸附检测试剂盒	1445
维生素D3(VD3)酶联免疫吸附检测试剂盒	1768
小鼠红细胞衍生核因子2样蛋白2(NFE2L2)酶联免疫吸附检测试剂盒	2025
小鼠Kelch样ECH关联蛋白1(KEAP1)酶联免疫吸附检测试剂盒	1819
大鼠内向整流型钾离子通道亚家族J成员10(KCNJ10)酶联免疫吸附检测试剂盒	1774
S-腺苷蛋氨酸(SAM)酶联免疫吸附检测试剂盒	2147
猪YY肽(PYY)酶联免疫吸附检测试剂盒	1519
牛纤溶酶原(Plg)酶联免疫吸附检测试剂盒	1862
牛43kDa胶原凝集素(CL43)酶联免疫吸附检测试剂盒	1880
猪铁调素(Hepc)酶联免疫吸附检测试剂盒	1452
溶血磷脂酸(LPA)酶联免疫吸附检测试剂盒	1582
环磷酸腺苷(cAMP)酶联免疫吸附检测试剂盒	2033
小鼠无翅型MMTV整合位点家族成员11(WNT11)酶联免疫吸附检测试剂盒	1223
白三烯C4(LTC4)酶联免疫吸附检测试剂盒	1205