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大鼠甲状腺过氧化物酶(TPO)酶联免疫吸附检测试剂盒

  • 货号:ELK2516
  • 价格:¥2,600.00 元
  • 点击:1784

产品介绍

产品信息

英文名称Rat TPO(Thyroid Peroxidase) ELISA Kit
别名TPX; thyroperoxidase

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Rat
检测类型Sandwich
灵敏度11.9 pg/mL
检测范围31.25-2000 pg/mL
样本类型serum, plasma and other biological fluids
实验时长3.5h

研究信息

研究领域Hematology;Hormone metabolism;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TPO. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TPO. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TPO, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TPO in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P14650

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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