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大鼠CD40配体(CD40L)酶联免疫吸附检测试剂盒

  • 货号:ELK2526
  • 价格:¥2,400.00 元
  • 点击:614

产品介绍

产品信息

英文名称Rat CD40L(Cluster Of Differentiation 40 Ligand) ELISA Kit
别名CD154; TRAP; HIGM1; IGM; IMD3; TBAM; T-BAM; TNFSF5; Gp39; TNF Superfamily Member 5; Hyper-IgM Syndrome; TNF-Related Activation Protein; T-Cell B-Cell Activating Molecule

规格与价格

48T 价格1680
96T 价格2400
96T*5 价格10200

检测参数

种属Rat
检测类型Sandwich
灵敏度0.052 ng/mL
检测范围0.16-10 ng/mL
样本类型serum, plasma, tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;CD & Adhesion molecule;Apoptosis;Tumor immunity;Infection immunity;Immune molecule;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CD40L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CD40L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CD40L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CD40L in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q9Z2V2

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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