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人碱性鞘磷脂磷酸二酯酶(Alk-Smase)酶联免疫吸附检测试剂盒

  • 货号:ELK2631
  • 价格:¥2,600.00 元
  • 点击:1930

产品介绍

产品信息

英文名称Human Alk-Smase(Alkaline Sphingomyelinase) ELISA Kit
别名ENPP7; E-NPP 7; NPP-7; Ectonucleotide Pyrophosphatase/Phosphodiesterase 7; Sphingomyelin Phosphodiesterase; Intestinal alkaline sphingomyelinase

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度0.109 ng/mL
检测范围0.32-20 ng/mL
样本类型tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;Neuro science;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Alk-Smase. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Alk-Smase. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Alk-Smase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Alk-Smase in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q6UWV6

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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