人骨成型蛋白7(BMP7)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human BMP7(Bone Morphogenetic Protein 7) Microsample ELISA Kit","alternative_names":"OP1; Osteogenic Protein-1; Eptotermin alfa","species":"Human","assay_type":"Sandwich","sensitivity":"27.6 pg\/mL","standard":"4000 pg\/mL","detection_range":"62.5-4000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Metabolic pathway;Developmental science;Bone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMP7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMP7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMP7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMP7 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P18075","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK2635MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK2635MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK2635MS",}