人Smad同源物4(Smad4)酶联免疫吸附检测试剂盒

英文名称	Human Smad4(Mothers Against Decapentaplegic Homolog 4) ELISA Kit
别名	DPC4; JIP; MADH4; Deletion target in pancreatic carcinoma 4

种属	Human
检测类型	Sandwich
灵敏度	0.113 ng/mL
检测范围	0.32-20 ng/mL
样本类型	Tissue homogenates, cell lysates and other biological fluids
实验时长	3.5h

研究领域	Signal transduction;Developmental science;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Smad4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Smad4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Smad4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Smad4 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q13485

标准曲线图

【仅供科学研究使用】

相关产品:

产品名称	点击数
人白介素2受体γ(IL2Rg)微量上样酶联免疫吸附检测试剂盒	1951
小鼠干扰素调节因子5(IRF5)酶联免疫吸附检测试剂盒	2086
小鼠Toll样受体1(TLR1)酶联免疫吸附检测试剂盒	1465
小鼠Toll样受体1(TLR1)微量上样酶联免疫吸附检测试剂盒	2084
人神经丝束蛋白(NPTN)酶联免疫吸附检测试剂盒	2091
人神经丝束蛋白(NPTN)微量上样酶联免疫吸附检测试剂盒	2182
人谷胱甘肽过氧化酶2(GPX2)酶联免疫吸附检测试剂盒	1708
人果糖二磷酸醛缩酶B(ALDOB)酶联免疫吸附检测试剂盒	1836
人钙/钙调蛋白依赖性蛋白激酶Ⅱβ(CAMK2b)酶联免疫吸附检测试剂盒	1835
人磷酯酶Cζ1(PLCz1)酶联免疫吸附检测试剂盒	2066
人前蛋白转化酶枯草溶菌素2(PCSK2)酶联免疫吸附检测试剂盒	2354
人前蛋白转化酶枯草溶菌素2(PCSK2)微量上样酶联免疫吸附检测试剂盒	2167
人L-型氨基酸转运蛋白1(y+LAT1)酶联免疫吸附检测试剂盒	2085
人钠耦合中性氨基酸转运蛋白1(SNAT1)酶联免疫吸附检测试剂盒	1327
人组蛋白脱乙酰基酶5(HDAC5)酶联免疫吸附检测试剂盒	2326
人核因子I/B(NFIB)酶联免疫吸附检测试剂盒	1491
人芳基硫酸酯酶A(ARSA)酶联免疫吸附检测试剂盒	2057
人GA结合蛋白转录因子α(GABPa)酶联免疫吸附检测试剂盒	2086
人SH3域结合蛋白2(SH3BP2)酶联免疫吸附检测试剂盒	1872
人苹果酸脱氢酶2(MDH2)酶联免疫吸附检测试剂盒	1956