人摄食抑制因子1(NES1)酶联免疫吸附检测试剂盒
- 货号：ELK2828
- 价格：￥2,600.00 元
- 点击：2020
产品介绍
产品信息
| 英文名称 | Human NES1(Nesfatin 1) ELISA Kit |
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| 别名 | Nesfatin-1 |
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规格与价格
| 48T 价格 | 1820 |
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| 96T 价格 | 2600 |
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| 96T*5 价格 | 11050 |
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检测参数
| 种属 | Human |
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| 检测类型 | Competitive Inhibition |
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| 灵敏度 | 225.6 pg/mL |
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| 检测范围 | 781.25-50000 pg/mL |
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| 样本类型 | serum, plasma and other biological fluids |
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| 实验时长 | 2h |
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研究信息
| 研究领域 | Glycolipid metabolism |
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| 检测原理 | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NES1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NES1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NES1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
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标准曲线
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