小鼠鳞状细胞癌抗原2(SCCA2)酶联免疫吸附检测试剂盒

英文名称	Mouse SCCA2(Squamous Cell Carcinoma Antigen 2) ELISA Kit
别名	SERPINB4; PI11; SPB4; Leupin; Serpin Peptidase Inhibitor,Clade B,Member 4; Peptidase Inhibitor 11

种属	Mouse
检测类型	Sandwich
灵敏度	0.056 ng/mL
检测范围	0.16-10 ng/mL
样本类型	Tissue homogenates, cell lysates and other biological fluids.
实验时长	3.5h

研究领域	Tumor immunity;Gastroenterology;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SCCA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SCCA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SCCA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SCCA2 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q9Z2G2

标准曲线图

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