人囊性纤维化跨膜传导调节因子(CFTR)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human CFTR(Cystic Fibrosis Transmembrane Conductance Regulator) ELISA Kit","alternative_names":"ABC35; ABCC7; CF; CFTR\/MRP; MRP7; TNR-CFTR; ATP-Binding Cassette Subfamily C,Member 7; Channel conductance-controlling ATPase; cAMP-dependent chloride channel","species":"Human","assay_type":"Sandwich","sensitivity":"0.06 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Immune molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFTR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFTR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFTR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFTR in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P13569","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK2973-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK2973-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK2973",}