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人VI型胶原(COL6)微量上样酶联免疫吸附检测试剂盒

  • 货号:ELK2977MS
  • 价格:¥2,600.00 元
  • 点击:2364
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产品介绍

产品信息

英文名称Human COL6(Collagen Type VI) Microsample ELISA Kit
别名collagen 6; OPLL ; PP3610

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度2.8 ng/mL
检测范围7.82-500 ng/mL
样本类型serum, plasma, tissue homogenates and other biological fluids
实验时长3.5h

研究信息

研究领域Metabolic pathway;Developmental science;Nutrition metabolism;Bone metabolism;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL6 in the samples is then determined by comparing the OD of the samples to the standard curve.

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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