| 英文名称 | Rat PON1(Paraoxonase 1) ELISA Kit |
|---|---|
| 别名 | ESA; PON; Esterase A; Serum paraoxonase/arylesterase 1; Aromatic esterase 1; A-esterase 1; Serum aryldialkylphosphatase 1 |
| 48T 价格 | 1820 |
|---|---|
| 96T 价格 | 2600 |
| 96T*5 价格 | 11050 |
| 种属 | Rat |
|---|---|
| 检测类型 | Sandwich |
| 灵敏度 | 0.064 ng/mL |
| 检测范围 | 0.16-10 ng/mL |
| 样本类型 | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| 实验时长 | 3.5h |
| 研究领域 | Metabolic pathway;Endocrinology; |
|---|---|
| 检测原理 | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PON1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PON1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PON1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PON1 in the samples is then determined by comparing the OD of the samples to the standard curve. |
| UniProt编号 | P55159 |
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