人Gremlin 1蛋白(GREM1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human GREM1(Gremlin 1) ELISA Kit","alternative_names":"PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1","species":"Human","assay_type":"Sandwich","sensitivity":"55 pg\/mL","standard":"8000 pg\/mL","detection_range":"125-8000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, urine and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;Tumor immunity;Developmental science;Bone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GREM1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GREM1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GREM1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GREM1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"O60565","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK3100-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK3100-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK3100",}