人丝氨酸肽酶抑制因子Kazal型1(SPINK1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human SPINK1(Serine Peptidase Inhibitor Kazal Type 1) ELISA Kit","alternative_names":"PCTT; PSTI; TATI; Tumor-Associated Trypsin Inhibitor; Pancreatic secretory trypsin inhibitor; Tumor-associated trypsin inhibitor","species":"Human","assay_type":"Sandwich","sensitivity":"0.61 ng\/mL","standard":"100 ng\/mL","detection_range":"1.57-100 ng\/mL","sample_type":"Serum, plasma and other biological fluids","assay_length":"3.5h","research_area":"Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SPINK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SPINK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SPINK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SPINK1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P00995","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK3201-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK3201-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK3201",}