人胃内因子(GIF)酶联免疫吸附检测试剂盒

英文名称	Human GIF(Gastric Intrinsic Factor) ELISA Kit
别名	TCN3; IF; IFMH; INF; Transcobalamin III; Vitamin B Synthesis

种属	Human
检测类型	Competitive Inhibition
灵敏度	0.91 ng/mL
检测范围	3.13-200 ng/mL
样本类型	Serum, plasma and other biological fluids
实验时长	2h

研究领域	Endocrinology;
检测原理	This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human GIF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GIF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GIF in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	P27352

标准曲线图

【仅供科学研究使用】

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