人Ⅻ型胶原(COL12)微量上样酶联免疫吸附检测试剂盒

英文名称	Human COL12(Collagen Type XII) Microsample ELISA Kit
别名	COL12-A1; COL12A1L; COL12A1; Collagen Alpha-1(XII)chain

种属	Human
检测类型	Sandwich
灵敏度	0.122 ng/mL
检测范围	0.32-20 ng/mL
样本类型	serum, plasma, tissue homogenates and other biological fluids
实验时长	3.5h

研究领域	Metabolic pathway;Tumor immunity;Bone metabolism;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL12 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	Q5KU26

标准曲线图

【仅供科学研究使用】

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