小鼠VGF神经生长因子诱导蛋白(VGF)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Mouse VGF(VGF Nerve Growth Factor Inducible) ELISA Kit","alternative_names":"NERP1; NERP2; Neuro-Endocrine Specific Protein VGF; Neuroendocrine Regulatory Peptide","species":"Mouse","assay_type":"Sandwich","sensitivity":"12.7 pg\/mL","standard":"2000 pg\/mL","detection_range":"31.25-2000 pg\/mL","sample_type":"serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Metabolic pathway;Infection immunity;Neuro science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VGF in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK3366-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK3366-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK3366",}