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人17-β-羟基类固醇脱氢酶14(HSD17b14)酶联免疫吸附检测试剂盒

  • 货号:ELK3584
  • 价格:¥2,600.00 元
  • 点击:2714
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产品介绍

产品信息

英文名称Human HSD17b14(17-Beta-Hydroxysteroid Dehydrogenase Type 14) ELISA Kit
别名DHRS10; RetSDR3, SDR47C1; Retinal Short-Chain Dehydrogenase/Reductase 3; Short Chain Dehydrogenase/Reductase Family 47C,Member 1

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度0.054 ng/mL
检测范围0.16-10 ng/mL
样本类型tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域Enzyme & Kinase;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSD17b14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSD17b14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSD17b14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSD17b14 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号Q9BPX1

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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