人SRC/FGR/YES相关FYN癌基因(FYN)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human FYN(FYN Oncogene Related To SRC\/FGR\/YES) ELISA Kit","alternative_names":"SLK; SYN; c-Fyn; Src-like kinase","species":"Human","assay_type":"Sandwich","sensitivity":"0.06 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids.","assay_length":"3.5h","research_area":"Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FYN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FYN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FYN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FYN in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK3670-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK3670-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK3670",}