人脑富含膜附着信号蛋白1(BASP1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human BASP1(Brain Abundant, Membrane Attached Signal Protein 1) ELISA Kit","alternative_names":"CAP-23; NAP-22; Brain acid soluble protein 1; 22 kDa neuronal tissue-enriched acidic protein; Neuronal axonal membrane protein NAP-22","species":"Human","assay_type":"Sandwich","sensitivity":"0.111 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BASP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BASP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BASP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BASP1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P80723","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK3743-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK3743-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK3743",}