人旁血小板溶蛋白(PPL)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human PPL(Periplakin) ELISA Kit","alternative_names":"190 kDa paraneoplastic pemphigus antigen; 195 kDa cornified envelope precursor protein","species":"Human","assay_type":"Sandwich","sensitivity":"0.126 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Metabolic pathway;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PPL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PPL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PPL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PPL in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"O60437","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK3840-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK3840-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK3840",}