人肽酰甘氨酸α酰胺化单加氧酶(PAM)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human PAM(Peptidylglycine Alpha Amidating Monooxygenase) ELISA Kit","alternative_names":"PAL; PHM; Peptidylami犬lycolate lyase; Peptidyl-Alpha-hydroxyglycine Alpha-amidating Lyase; Peptidylglycine Alpha-Hydroxylating Monooxygenase","species":"Human","assay_type":"Sandwich","sensitivity":"14.5 pg\/mL","standard":"2000 pg\/mL","detection_range":"31.25-2000 pg\/mL","sample_type":"serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAM in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P19021","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK3953-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK3953-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK3953",}