人磷脂酶D(PLD)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human PLD(Phospholipase D) Microsample ELISA Kit","alternative_names":"PLD1; Choline Phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1","species":"Human","assay_type":"Sandwich","sensitivity":"1.42 ng\/mL","standard":"200 ng\/mL","detection_range":"3.13-200 ng\/mL","sample_type":"Serum, plasma and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;Enzyme & Kinase;Metabolic pathway;Nutrition metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLD in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q13393","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4037MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4037MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4037MS",}