人亚甲基四氢叶酸还原酶(MTHFR)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human MTHFR(Methylenetetrahydrofolate Reductase) ELISA Kit","alternative_names":"5,10-Methylenetetrahydrofolate Reductase(NADPH)","species":"Human","assay_type":"Sandwich","sensitivity":"22.9 pg\/mL","standard":"4000 pg\/mL","detection_range":"62.5-4000 pg\/mL","sample_type":"serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MTHFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTHFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MTHFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MTHFR in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P42898","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4174-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4174-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4174",}