人黑色素瘤优先表达抗原(PRAME)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human PRAME(Preferentially Expressed Antigen In Melanoma) ELISA Kit","alternative_names":"MAPE; OIP4; CT130; Cancer\/Testis Antigen 130; Opa-interacting protein 4; Melanoma antigen preferentially expressed in tumors","species":"Human","assay_type":"Sandwich","sensitivity":"0.063 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PRAME. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PRAME. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PRAME, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PRAME in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P78395","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4314-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4314-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4314",}