当前位置:首页>产品中心>Elisa试剂盒>人FK506结合蛋白12雷帕霉素关联蛋白(FRAP)酶联免疫吸附检测试剂盒

人FK506结合蛋白12雷帕霉素关联蛋白(FRAP)酶联免疫吸附检测试剂盒

  • 货号:ELK4316
  • 价格:¥2,600.00 元
  • 点击:1716

产品介绍

产品信息

英文名称Human FRAP(FK506 Binding Protein 12 Rapamycin Associated Protein) ELISA Kit
别名FRAP1; MTOR; FRAP2; MTOR; RAFT1; RAPT1; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target 1

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Human
检测类型Sandwich
灵敏度0.116 ng/mL
检测范围0.32-20 ng/mL
样本类型tissue homogenates, cell lysates and other biological fluids
实验时长3.5h

研究信息

研究领域Tumor immunity;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FRAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FRAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FRAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FRAP in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P42345

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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