人G蛋白偶联受体78(GPR78)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Human GPR78(G Protein Coupled Receptor 78) Microsample ELISA Kit","alternative_names":"G protein coupled receptor GPR78; G-protein coupled receptor 78; GPR78","species":"Human","assay_type":"Sandwich","sensitivity":"0.065 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"CD & Adhesion molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPR78. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPR78. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPR78, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPR78 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q96P69","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4387MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4387MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4387MS",}