人小异二聚体伴侣(SHP)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human SHP(Small Heterodimer Partner) ELISA Kit","alternative_names":"NR0B2; SHP1; Nuclear Receptor Subfamily 0,Group B,Member 2; Orphan nuclear receptor SHP","species":"Human","assay_type":"Sandwich","sensitivity":"0.055 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;Endocrinology;Developmental science;Hormone metabolism;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SHP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SHP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SHP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SHP in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q15466","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4427-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4427-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4427",}