小鼠CD14分子(CD14)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Mouse CD14(Cluster Of Differentiation 14) Microsample ELISA Kit","alternative_names":"Monocyte differentiation antigen CD14; Myeloid cell-specific leucine-rich glycoprotein","species":"Mouse","assay_type":"Sandwich","sensitivity":"0.141 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids.","assay_length":"3.5h","research_area":"CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CD14. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CD14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CD14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CD14 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P10810","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4475MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4475MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4475MS",}