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大鼠蛋白激酶Cε(PKCe)酶联免疫吸附检测试剂盒

  • 货号:ELK4533
  • 价格:¥2,600.00 元
  • 点击:1008

产品介绍

产品信息

英文名称Rat PKCe(Protein Kinase C Epsilon) ELISA Kit
别名PKC-E; PRKCE; PRKC-E; NPKC-Epsilon

规格与价格

48T 价格1820
96T 价格2600
96T*5 价格11050

检测参数

种属Rat
检测类型Sandwich
灵敏度0.57 ng/mL
检测范围1.57-100 ng/mL
样本类型tissue homogenates, cell lysates, cell culture supernates and other biological fluids
实验时长3.5h

研究信息

研究领域Signal transduction;Enzyme & Kinase;Apoptosis;Endocrinology;Cardiovascular biology;
检测原理The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PKCe. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PKCe. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PKCe, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PKCe in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号P09216

标准曲线

标准曲线图标准曲线图
【仅供科学研究使用】

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