人B-细胞淋巴瘤因子11A(Bcl11A)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human Bcl11A(B-Cell CLL\/Lymphoma 11A) ELISA Kit","alternative_names":"BCL11A-L; BCL11A-S; BCL11A-XL; CTIP1; EVI9; Ecotropic Viral Integration Site 9; COUP-TF-interacting protein 1; Ecotropic viral integration site 9 homolog; Zinc finger protein 856","species":"Human","assay_type":"Sandwich","sensitivity":"0.118 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Immune molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Bcl11A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Bcl11A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Bcl11A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Bcl11A in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9H165","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4535-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4535-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4535",}