人G蛋白偶联受体109B(GPR109B)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Human GPR109B(G Protein Coupled Receptor 109B) ELISA Kit","alternative_names":"HCAR3; NIACR2; HM74; HCA3; HM74B; PUMAG; Puma-g; Niacin Receptor 2; Nicotinic acid receptor 2; Hydroxycarboxylic acid receptor 3","species":"Human","assay_type":"Sandwich","sensitivity":"0.062 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids.","assay_length":"3.5h","research_area":"CD & Adhesion molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPR109B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPR109B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPR109B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPR109B in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P49019","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4567-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4567-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4567",}