人含T-细胞免疫球蛋白粘蛋白域蛋白4(TIMD4)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human TIMD4(T-Cell Immunoglobulin And Mucin Domain Containing Protein 4) Microsample ELISA Kit","alternative_names":"TIM4; T-cell membrane protein 4; T-cell immunoglobulin mucin receptor 4","species":"Human","assay_type":"Sandwich","sensitivity":"0.057 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human TIMD4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human TIMD4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human TIMD4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human TIMD4 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q96H15","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4680MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4680MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4680MS",}