人L-型氨基酸转运蛋白1(y+LAT1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human y+LAT1(L-Type Amino Acid Transporter 1) ELISA Kit","alternative_names":"SLC7A7; LPI; Y+LAT1; MOP-2; Monocyte amino acid permease 2; Y+L Amino Acid Transporter 1; Solute Carrier Family 7 Member 7,Cationic Amino Acid Transporter,y+ System","species":"Human","assay_type":"Sandwich","sensitivity":"0.118 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human y+LAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human y+LAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human y+LAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human y+LAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9UM01","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4710-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4710-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4710",}