人钠耦合中性氨基酸转运蛋白1(SNAT1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human SNAT1(Sodium Coupled Neutral Amino Acid Transporter 1) ELISA Kit","alternative_names":"SLC38A1; NAT2; SAT1; ATA1; Solute Carrier Family 38 Member 1; N-system amino acid transporter 2; Amino acid transporter A1; System N amino acid transporter 1","species":"Human","assay_type":"Sandwich","sensitivity":"0.055 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids.","assay_length":"3.5h","research_area":"Signal transduction;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SNAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SNAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SNAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SNAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"Q9H2H9","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4711-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4711-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4711",}