人前胶原赖氨酸-2-酮戊二酸-5-双加氧酶1(PLOD2)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human PLOD2(Procollagen Lysine-2-Oxoglutarate-5-Dioxygenase 2) ELISA Kit","alternative_names":"TLH; LH2; Telopeptide Lysyl Hydroxylase; Lysyl hydroxylase 2","species":"Human","assay_type":"Sandwich","sensitivity":"0.059 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids.","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PLOD2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PLOD2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PLOD2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PLOD2 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"O00469","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4865-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4865-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4865",}