人FK506结合蛋白1B(FKBP1B)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human FKBP1B(FK506 Binding Protein 1B) ELISA Kit","alternative_names":"FKBP12.6; FKBP1L; FKBP9; OTK4; PKBP1L; Rotamase; 12.6 kDa FK506-binding protein; Immunophilin FKBP12.6; Peptidyl-prolyl cis-trans isomerase FKBP1B","species":"Human","assay_type":"Sandwich","sensitivity":"0.054 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids.","assay_length":"3.5h","research_area":"Immune molecule;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FKBP1B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FKBP1B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FKBP1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FKBP1B in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P68106","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK4956-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK4956-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK4956",}