人乳腺癌抗雌激素药物耐药性基因3(BCAR3)酶联免疫吸附检测试剂盒

英文名称	Human BCAR3(Breast Cancer Anti-Estrogen Resistance 3) ELISA Kit
别名	NSP2; SH2D3B; Novel SH2-containing protein 2; SH2 domain-containing protein 3B

种属	Human
检测类型	Sandwich
灵敏度	28 pg/mL
检测范围	78.13-5000 pg/mL
样本类型	Serum, plasma, tissue homogenates and other biological fluids.
实验时长	3.5h

研究领域	Tumor immunity;
检测原理	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BCAR3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BCAR3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BCAR3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BCAR3 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt编号	O75815

标准曲线图

【仅供科学研究使用】

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