人血管内皮生长因子抑制因子(VEGI)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human VEGI(Vascular Endothelial Growth Inhibitor) ELISA Kit","alternative_names":"TNFSF15; TL1; TL1A; VEGI192A; Tumor Necrosis Factor(ligand)Superfamily,Member 15; TNF Superfamily Ligand TL1A; TNF Ligand-Related Molecule 1","species":"Human","assay_type":"Sandwich","sensitivity":"27.1 pg\/mL","standard":"4000 pg\/mL","detection_range":"62.5-4000 pg\/mL","sample_type":"serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Tumor immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human VEGI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human VEGI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human VEGI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human VEGI in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"O95150","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5057-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5057-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5057",}