人肝配蛋白A2(EFNA2)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human EFNA2(Ephrin A2) Microsample ELISA Kit","alternative_names":"ELF-1; EPLG6; HEK7-L; LERK6; EPH-related receptor tyrosine kinase ligand 6; HEK7 ligand","species":"Human","assay_type":"Sandwich","sensitivity":"0.054 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"Tissue homogenates, cell lysates and other biological fluids.","assay_length":"3.5h","research_area":"Neuro science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EFNA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EFNA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EFNA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EFNA2 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"O43921","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5128MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5128MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5128MS",}