人磷脂酰肌醇-4-激酶α(PI4Ka)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human PI4Ka(Phosphatidylinositol-4-Kinase Catalytic Alpha) ELISA Kit","alternative_names":"PIK4CA; PI4K-ALPHA; pi4K230","species":"Human","assay_type":"Sandwich","sensitivity":"0.26 ng\/mL","standard":"50 ng\/mL","detection_range":"0.79-50 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PI4Ka. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PI4Ka. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PI4Ka, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PI4Ka in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P42356","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5139-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5139-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5139",}