{"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Rat FST(Follistatin) ELISA Kit","alternative_names":"FST; Activin-binding protein;FS; FST; Follistatin","species":"Rat","assay_type":"Competitive Inhibition","sensitivity":"0.53 ng\/mL","standard":"100 ng\/mL","detection_range":"1.57-100 ng\/mL","sample_type":"serum, plasma, other biological fluids","assay_length":"2h","research_area":"Endocrinology;Reproductive science;Hormone metabolism;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat FST. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FST in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P21674","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5276-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5276-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5276",}