人泛素(Ub)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human Ub(Ubiquitin) ELISA Kit","alternative_names":"Ubiquitin B; UbB; Ubiquitous Immunopoietic Polypeptide; Polyubiquitin B","species":"Human","assay_type":"Sandwich","sensitivity":"2.53 ng\/mL","standard":"400 ng\/mL","detection_range":"6.25-400 ng\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"Reproductive science;Neuro science;Developmental science;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Ub. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Ub. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Ub, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Ub in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5287-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5287-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5287",}