小鼠安眠蛋白1α(MEP1a)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Mouse MEP1a(Meprin A Alpha) ELISA Kit","alternative_names":"PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase","species":"Mouse","assay_type":"Competitive Inhibition","sensitivity":"19.1 pg\/mL","standard":"4000 pg\/mL","detection_range":"62.5-4000 pg\/mL","sample_type":"Serum, plasma, cell lysates, cell culture supernates and other biological fluids","assay_length":"2h","research_area":"Metabolic pathway;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse MEP1a. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MEP1a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MEP1a in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P28825","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5307-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5307-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5307",}