小鼠趋化因子C-C-基元受体2(CCR2)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1680","96t":"2400","96t_5":"10200","name_en":"Mouse CCR2(Chemokine C-C-Motif Receptor 2) Microsample ELISA Kit","alternative_names":"CD192; CC-CKR2; CCR2A; C-C CKR-2; CCR2B; CKR2-A; CKR2-B; CMKBR2; MCP1-R; Monocyte chemoattractant protein 1 receptor","species":"Mouse","assay_type":"Sandwich","sensitivity":"32 pg\/mL","standard":"5000 pg\/mL","detection_range":"78.13-5000 pg\/mL","sample_type":"serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids","assay_length":"3.5h","research_area":"CD & Adhesion molecule;Tumor immunity;Infection immunity;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CCR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CCR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CCR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CCR2 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P51683","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5338MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5338MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5338MS",}