人内皮抑素(ES)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human ES(Endostatin) ELISA Kit","alternative_names":"FLJ27325; FLJ34914; KNO; KNO1; MGC74745;","species":"Human","assay_type":"Competitive Inhibition","sensitivity":"1.72 ng\/mL","standard":"400 ng\/mL","detection_range":"6.25-400 ng\/mL","sample_type":"serum, plasma, cell lysates, cell culture supernates and other biological fluids","assay_length":"2h","research_area":"Tumor immunity;Cardiovascular biology;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human ES. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ES. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ES in the samples is then determined by comparing the OD of the samples to the standard curve.","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5401-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5401-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5401",}