牛转化生长因子β1(TGFb1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Cattle TGFb1(Transforming Growth Factor Beta 1) ELISA Kit","alternative_names":"TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide","species":"Cattle","assay_type":"Competitive Inhibition","sensitivity":"4.8 pg\/mL","standard":"1000 pg\/mL","detection_range":"15.63-1000 pg\/mL","sample_type":"serum, platelet-poor plasma, cell culture supernates and other biological fluids","assay_length":"2h","research_area":"Cytokine;Tumor immunity;Infection immunity;","test_principle":"This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P18341","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5467-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5467-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5467",}