人蛋白酶体亚基β10(PSMb10)微量上样酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1820","96t":"2600","96t_5":"11050","name_en":"Human PSMb10(Proteasome Subunit Beta Type 10) Microsample ELISA Kit","alternative_names":"LMP10; MECL1; Low molecular mass protein 10; Macropain subunit MECl-1; Multicatalytic endopeptidase complex subunit MECl-1; Proteasome subunit beta-2i","species":"Human","assay_type":"Sandwich","sensitivity":"0.115 ng\/mL","standard":"20 ng\/mL","detection_range":"0.32-20 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMb10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSMb10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSMb10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMb10 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P40306","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5601MS-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5601MS-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5601MS",}