大鼠防御素β1(DEFb1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat DEFb1(Defensin Beta 1) ELISA Kit","alternative_names":"B-DF1; DEFB1; BD1; DEF-B1; DEFB101; HBD1","species":"Rat","assay_type":"Sandwich","sensitivity":"19.52 pg\/mL","standard":"3000 pg\/mL","detection_range":"46.88-3000 pg\/mL","sample_type":"Serum, plasma, tissue homogenates and other biological fluids","assay_length":"3.5h","research_area":"Infection immunity;Hepatology;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DEFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DEFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DEFb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DEFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"O89117","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5637-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5637-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5637",}