大鼠AMP激活蛋白激酶β1(PRKAb1)酶联免疫吸附检测试剂盒

产品介绍

• {"48t":"1960","96t":"2800","96t_5":"11900","name_en":"Rat PRKAb1(Protein Kinase, AMP Activated Beta 1) ELISA Kit","alternative_names":"AMPK; HAMPKb; 5'-AMP-Activated Protein Kinase Subunit Beta-1 Non-Catalytic Subunit; Adenosine Monophosphate Activated Protein Kinase(AMPK)","species":"Rat","assay_type":"Sandwich","sensitivity":"0.056 ng\/mL","standard":"10 ng\/mL","detection_range":"0.16-10 ng\/mL","sample_type":"tissue homogenates, cell lysates and other biological fluids","assay_length":"3.5h","research_area":"Enzyme & Kinase;Metabolic pathway;","test_principle":"The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat PRKAb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PRKAb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat PRKAb1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PRKAb1 in the samples is then determined by comparing the OD of the samples to the standard curve.","uniprot_id":"P80386","StandardCurveURL":"https:\/\/www.elkbiotech.cn\/upload\/image\/ELISA\/ELK5668-b-1.jpg","ManualURL":"https:\/\/www.elkbiotech.cn\/upload\/file\/ELISA\/ELK5668-1.pdf","ProductURL":"https:\/\/www.elkbiotech.cn\/pro\/ELK5668",}